|
KMID : 0545120170270071276
|
|
Journal of Microbiology and Biotechnology
2017 Volume.27 No. 7 p.1276 ~ p.1280
|
|
|
Generation of Newly Discovered Resistance Gene mcr-1 Knockout in Escherichia coli Using the CRISPR/Cas9 System
|
|
Sun Lichang
He Tao
Zhang Lili
Pang Maoda
Zhang Qiaoyan
Zhou Yan
Bao Hongduo
Wang Ran
|
|
|
Abstract
|
|
|
|
The mcr-1 gene is a new “superbug” gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The mcr-1 gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce mcr-1 gene knockout in Escherichia coli. Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene mcr-1 might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas::mcr targeting specific DNA sequences of the mcr-1 gene into microbial populations.
|
|
|
KEYWORD
|
|
|
mcr-1, knockout, E. coli, CRISPR/Cas9 system, BMAP-27
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|